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            1. Reagent Center

              Animal Pathogen Series

              • CSFV Real Time RT-PCR Detection kit MORE
                Product Details:

                This kit uses RNA as a template to transcribe and synthesize cDNA strands under the action of reverse transcriptase. Under the action of hot-start polymerase, the qualitative detection of classical swine fever virus is achieved through polymerase chain reaction (PCR) combined with Taqman fluorescent probe technology. The primers and Taqman probes in this kit are designed with specific fragments of classical swine fever virus, which are suitable for auxiliary diagnosis, detection and epidemiological investigation of classical swine fever virus (CSFV) in pig tissues and blood.

                The kit is suitable for all kinds of real-time quantitative detection systems, and is especially suitable for the Line-Gene series fluorescent quantitative PCR detection system of Hangzhou Bioer Technology Co., Ltd

                 

                Cat#

                BSL28S1

                BSL28M1

                Components

                24 T

                48 T

                CSFV RT-PCR Buffer

                360 µL

                720 µL

                CSFV Positive control

                200 µL

                200 µL

                CSFV Negative control

                200 µL

                200 µL

                Storage conditions and expiry date

                1. The kit must be shipped under refrigerated conditions.

                2. Preservation of the kit: Store in a refrigerator at -20°C in the dark. Repeated freezing and thawing should not exceed 5 times.

                3. Validity period: 12 months, please use within the validity period

                Product Features:

                1.High specificity: design primers and probes for classic swine fever virus

                2.Wide adaptability: It can be used with Hangzhou Bioer Line-Gene series fluorescent quantitative PCR detection system and similar fluorescent quantitative PCR detection systems of other manufacturers

                3.Simple operation and short time-consuming: Pre-mixed reagents are used, with few components and convenient operation

                4.The results are objective and reliable: use the Ct value to automatically quantify the sample concentration

                Experimental data:

                Use MagaBio plus Virus DNA/RNA Purification Kit II to extract samples containing classical swine fever virus, and perform sensitivity verification after 10-fold serial dilution.

                Result

                CSFV

                Ct Value

                Original liquid

                18.66

                18.51

                10 times dilution

                22.29

                22.28

                100 times dilution

                28.82

                25.7

                1000 times dilution

                29.39

                29.57

                10000 times dilution

                32.96

                32.62

                100000 times dilution

                35.62

                35.77

                Negative control

                NoCt

                NoCt

                 

                 

                 

                 

                 

                Manual download
              • ASFV Wild Strain and Gene-deleted Vaccine Strain Real Time PCR Dual Detection kit MORE
                Product Details:

                This kit selects specific fragments in the gene sequence of the African swine fever virus wild strain and gene-deleted vaccine strain to design primers and Taqman probes, and through polymerase chain reaction (PCR) combined with Taqman fluorescent probe technology to achieve qualitative detection of African swine fever virus wild strains and gene deletion vaccine strains.

                 

                Cat# 

                BSL27S1

                BSL27M1

                Components

                24 T

                48 T

                2×PCR Buffer

                480 µL

                960 µL

                Positive comtrol

                200 µL

                200 µL

                Negative control

                200 µL

                200 µL

                Storage conditions and expiry date

                1. The kit must be shipped under refrigerated conditions.

                2. Preservation of the kit: Store in a refrigerator at -20°C in the dark. Repeated freezing and thawing should not exceed 5 times.

                3. Validity period: 12 months, please use within the validity period

                Product Features:
                1. Wide adaptability: open reagents, suitable for various quantitative PCR instruments, especially for related quantitative PCR instruments of Bioer company
                2. Simple operation and short time-consuming: Pre-mixed reagents are used, with few components and convenient operation
                3. Objective and reliable results: use Ct value to automatically qualitative samples
                Experimental data:

                Case 1

                Perform sensitivity verification after serial dilution with recombinant plasmids containing the target fragments of ASFV wild strain and gene deletion vaccine strain.

                Ct value

                Sample

                FAM

                HEX

                Sample 1

                38.72

                37.23

                Sample 2

                37.81

                36.33

                Positive

                25.37

                24.38

                Negative

                NoCt

                NoCt

                 

                Case 2

                Use MagaBio plus Virus DNA Purification Kit II to extract samples containing wild strains of African swine fever virus, and use the kit to test the extracted products. The results are as follows:

                Ct value

                Sample

                FAM

                HEX

                Sample 1

                38.72

                37.23

                Sample 2

                37.81

                36.33

                Positive

                25.37

                24.38

                Negative

                NoCt

                NoCt

                 

                Manual download
              • ASFV real-time PCR detection reagent MORE
                Product Details:

                African Swine fever virus (ASFV) is a double-stranded DNA virus belonging to Asfarviridae, which can cause acute and hemorrhagic symptoms in domestic pigs or wild boar after infection. The virus spreads quickly among pigs and could have serious effects for the breeding industry if it is not detected in time. Bioer ASFV real-time PCR detection reagent series is specially designed for this virus, with high efficiency, stability, strong specificity and other characteristics, can meet the needs of different users.

                Product Features:

                1.Short time: the detection time is short and can be used with Bioer one-step method virus nucleic acid extraction kit.

                2.Monitorable: the whole process of sampling, extraction and PCR detection can be monitored

                3.High efficiency and stability: specially designed for African swine fever virus, with good practicality, stable storage and transportation

                4.Strong versatility: Suitable for all kinds of PCR detection system

                Experimental data:

                Case 1

                Nucleic acid was extracted from pig whole blood samples containing African swine fever virus using Bioer MagaBio Plus virus DNA extraction kit II. After gradient dilution of nucleic acid (10-100,000 times), Bioer ASFV real-time PCR detection kit was used for detection. The results were shown in Figure 1 and Figure 2:

                figure 1

                Figure 2

                Case 2

                The nucleic acid containing ASFV samples was extracted with the Simply P virus DNA/RNA extraction kit of Bioer Technology, and then detected with the Bioer ASFV real-time PCR detection kit and the other detection reagent of C Company in the market. The results were shown in Figure 3 and Figure 4:

                Bioer Technology

                Figure 3

                 

                C company

                Figure 4

                The results showed that Bioer ASFV real-time PCR detection reagent detected one more sample than other reagents from C company, indicating that Bioer ASFV real-time PCR detection reagent kit is more sensitive and has a stronger gray area sample detection ability in use, which reduces the risk of missing detection.

                Manual download
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