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            1. Reagent Center

              Aquatic Pathogen Series

              • Aquatic Pathogen Series Reagents MORE
                Product Details:

                In this series of products, primers and TaqMan probes are designed with virus specific gene fragments to realize rapid qualitative detection of viral nucleic acids in aquatic and economic animals such as shrimp and fish. It can be used for virus tracking detection in the breeding process, and also for environmental monitoring, so as to avoid the spread of virus and reduce economic losses. The series also has multi-channel reagents that simultaneously detect two diseases in the same tube.

                 

                 

                Main component

                Storage temperature

                Amplification reagents

                PCR reaction reagents

                -15~-25℃

                Primers probe

                Control

                Negative control

                Positive control

                 

                Product list

                Cat#

                Product Name

                Package

                Note

                BSN01S1

                White Spot Syndrome Virus Real Time PCR Detection Kit

                16T

                -20℃Storage

                BSN01M1

                32T

                BSN02S1

                Infectious hypodermal and haematopoietic necrosis virus Real Time PCR Detection Kit

                16T

                BSN02M1

                32T

                BSN03S1

                Shrimp Hemocyte Iridescent Virus Real Time PCR Detection Kit

                16T

                BSN03M1

                32T

                BSN04S1

                Vibrio Harveyi Real Time PCR Detection Kit

                16T

                BSN04M1

                32T

                BSN05S1

                EMS & EHP Dual Chanel Real Time PCR Detection kit

                16T

                BSN05M1

                32T

                BSN06S1

                Bacteria & Vibrio Dual Chanel Real Time PCR Detection Kit

                16T

                BSN06M1

                32T

                BSN07S1

                Taura Syndrome Virus Real Time PCR Detection Kit

                16T

                BSN07M1

                32T

                BSN08S1

                Yellow head virus Real Time PCR Detection Kit

                16T

                BSN08M1

                32T

                BSN09S1

                Enterocytozoon hepatopenaei Real Time PCR Detection Kit

                16T

                BSN09M1

                32T

                BSN10S1

                Acute Hepatopancreatic Necrosis Disease Real Time PCR Detection Kit

                16T

                BSN10M1

                32T

                 


                Product Features:

                1. It is suitable for the Bioer fluorescence quantitative PCR detection system and the same kind of real-time quantitative detection system

                2. High sensitivity: detection limit up to 100 copies/mL

                3. High specificity: The design of specific primers and probes can safely avoid cross reactions and the resulting false positives

                4. Easy to operate, short time consuming: the reagent adopts premixed form, less components, easy to operate, only need about 1 hour to complete whole experiment.

                5. DUTP-UNG system is used to effectively prevent contamination of PCR products

                Experimental data:

                Experiment 1

                White Spot Syndrome Virus Real Time PCR Detection Kit

                (1) Linear tests with concentrations of 102 to 107 copies/mL of DNA showed good results, R2=0.9985

                DNA(copies/mL)

                Ct

                Ct value

                106

                19.18

                19.2

                19.22

                105

                22.64

                22.63

                22.62

                104

                26.31

                26.37

                26.43

                103

                29.82

                29.83

                29.84

                102

                34.1

                34.11

                34.12

                 

                 

                 

                 

                (2) The test was repeated using lower concentrations of DNA

                 

                The results showed that the detection rate of low concentration DNA was 100%, and the reproducibility was good


                Experiment 2

                 Dual nucleic acid detection reagent linear test of acute hepatopancreas necrosis (EMS) and hepatoenterocytic worm (EHP) (DNA concentration 102 ~ 107copies/mL)

                DNA(copies/mL)

                EHP(FAM)

                EMS(HEX)

                106

                16.93

                16.42

                105

                20.3

                19.95

                104

                24.12

                23.41

                103

                27.73

                27.22

                102

                31.62

                31.48

                 




































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